Description of a one step staggered reannealing method for directional cloning of PCR-generated dna using sticky-end ligation without employing restriction enzymes
نویسندگان
چکیده
منابع مشابه
Site-directed mutagenesis using a PCR-based staggered re-annealing method without restriction enzymes.
Am. J. Pathol. 143:1527-1534. 4.Haase, A.T., E.F. Retael and K.A. Staskus. 1990. Amplification and detection of lentiviral DNA inside cells. Proc. Natl. Acad. Sci. USA 87:4971-4975. 5.Janssen, K. (Ed). 1995. In situ polymerase chain reaction and hybridization to detect lowabundance nucleic acid targets, p. 14.8.114.8.24. Current Protocols in Molecular Biology, Vol. 2, Supplement 31. John Wiley ...
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ژورنال
عنوان ژورنال: IUBMB Life
سال: 1996
ISSN: 1521-6543
DOI: 10.1080/15216549600201861